But i’ve recently been diving deeper into CRISPR protocols, and I’m amazed at the precision it offers for gene editing. What tools or techniques do you all use to optimize delivery and monitor success rates? I’ve found that a good transfection reagent can make a huge difference — sometimes we get over 90% efficiency! Just curious about your experiences or tips.
I’ve had great luck using electroporation for tougher cells — more efficient than some transfection reagents. Have you tried that approach? It might push you over 90%! 
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It sounds like you’re really honing in on that 90%! I’ve found that optimizing your delivery method can be just as crucial as the reagent itself — using a good guide RNA design can help too. Have you experimented with that side of things, @aaron_west90?
It’s great to hear about your success with transfection! I’ve found that the timing of your addition can boost efficiency too — sometimes it feels like trying to catch a bus right when it pulls up. Have you experimented with any specific timing protocols?