The Day the PCR Went Rogue

So, the other day, we had a PCR run that decided to amplify every little speck of DNA it could find. We ended up with results that looked like a family reunion — way too many bands on the gel. It’s a reminder that in the lab, even the most controlled assays can have their quirks. Anyone else had a PCR meltdown?

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Sounds like your PCR threw a party! I had a similar experience once, but I learned to always include a negative control — it’s like bringing a bouncer to keep things in check. Ever thought about adjusting the MgCl2 concentration for those wild runs?

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