Interpreting enzyme assay results

I recently ran an enzyme activity assay using spectrophotometry and found some unexpected results. The absorbance was significantly lower than anticipated. Has anyone experienced similar issues when measuring enzyme kinetics, and how did you troubleshoot? It would be great to hear your insights or resource recommendations.

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If you’re seeing unexpected absorbance values, double-check your reagent concentrations and the timing of your measurements — timing can really skew results. It reminds me of when we faced similar issues, and recalibrating our pipettes made a world of difference. Have you tried adjusting those parameters?

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